Facts-enriched characterization of mobile populations. Morphological details from illustrations or photos can insert to your richness of flow cytometry details. Such as, the figure demonstrates an usually common apoptosis assay making use of Annexin V and PI, adding mobile imaging to characterize cells in Each and every population to reveal morphologically distinctive functions. These insights couldn't have already been acquired from multiplex staining by yourself.

Irrespective of whether you’re a starter or a specialist, examine our sources to progress your investigate and experience in flow cytometry.

(B) Uncooked 264.7 macrophages will often be annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The number of singlets obtainable for Investigation will increase once the person trains and employs a more accurate model.

Learn new insights into your experimental facts by visualizing populations making use of combined fluorescence and morphology info. Our computer software interface wizards allow downsampling, concatenation (merged data files) and dimensionality equipment which can be built to the application for an intuitive, streamlined data Examination experience.

"An image is just not quite really worth 1000 dots, nevertheless it actually helps to see visuals. It is excellent to discover illustrations or photos that correlate with staining. For instance, AnnexinV-positive cells which can be morphologically distinct than balanced cells, or CD14+ monocytes that happen to be more substantial and more textured than CD3+/CD4+/CD14- lymphocytes.

Also, it really is illuminating to find out how many doublets slip as a result of common singlet gates, and terrific to validate the position of surprising double-optimistic events in my Investigation."

Optimizing gating technique. Even sturdy handbook singlet gating is mistake-prone and continues to be a subjective determination position in Pretty much all flow cytometry assays. Imaging may be used to confirm and adjust gates to incorporate only one cells of interest.

Regular image good quality even at higher throughput Acoustic focusing plus a significant-velocity digicam combine to picture these T cells continually at low or substantial flow costs. Very easily adjust target and digital camera options to satisfy experimental necessities.

Early signs of this contamination experienced in the beginning been dismissed as debris. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also enables you to use morphological capabilities to discover exciting subpopulations that might not be obvious from flow cytometry information on your own.

End users can see party visuals in true-time to substantiate sample good quality, enhance protocols, and history the morphology of each cell population recognized for Evaluation. Imaging capture also Gains from acoustic focusing, which really helps to placement the cells to ensure that a sharp, centered image is obtained.

AI-pushed automatic image Examination Together with the Attune Cytometric Application translates celebration features into distinct morphology parameters which can be combined with common fluorescence and scatter parameters to expose hugely reproducible populace alterations and special subsets.

Application Take note: Automated image Examination cuts down consumer-to-person variability in flow cytometry gating procedures

Created-in dimensionality reduction applications elevated resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to confirm expected populace resolution in lysed full human blood. Consumer can watch situations from Every single cluster for Visible affirmation check here of cell phenotypes.

Adaptable payment program from Agilent Economical Methods let you receive the essential instrumentation you need for immediate task, or to grow your lab.

Gating on these extended parameters enables you to speedily and accurately discover populations of interest and make sure gating approach with little or no guide assessment. The Attune software package gives access to condition-of-the-art equipment Finding out Investigation tools in just an expertly built person interface, rendering it exceptionally easy to use.

As the cell line experienced Formerly been analyzed on the Attune CytPix Flow Cytometer, the researchers went again to the images and had been able to document the microbial an infection not less than 5 times previously.

The Attune CytPix Flow Cytometer lets buyers to visualize samples during acquisition, confirm the accuracy of gating tactics, and support final results with dependable documentation for publication reasons.

What seemed to be a homogeneous population is actually a lot more numerous—an insight that should be viewed as when interpreting success.